Study of water-borne coliform bacteria by use of desoxycholate agar and violet red bile agar.

Comparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods.

A 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 +/- 1 degrees C and transfer to 44 +/- 1 degrees C for 22 h) using fecal coliform agar (FCA) was compared with the 24-h standardized violet red bile lactose agar (VRBL) method. FCA and VRBL have equivalent specificities and sensitivities, except for lactose-positive non-fecal colif...

Performance comparison of the BioSys optical assay and the violet red bile agar method for detecting coliforms in food products.

Coliform counts in a variety of foods, including dairy products (raw milk, pasteurized milk, yogurt, butter, and ice cream), meats (pork sausage, ground beef, and raw chicken), raw eggs, and chocolate, were performed by the rapid automated BioSys optical assay and the conventional method with violet red bile agar (VRBA). The standard deviation (SD) among five replicate counts for the optical as...

Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae.

Bile-esculin agar has been used for several years for the presumptive identification of group D streptococci. All members of the Enterobacteriaceae family will also grow on this medium, but only certain ones can hydrolyze esculin to 6,7-dihydroxycoumarin, which reacts with iron to produce a characteristic blackening of the medium. One thousand and six cultures from clinical specimens representi...

Suitability of MRS-bile Agar for the Selective Enumeration of Mixed Probiotic Bacteria in Presence of Mesophilic Lactic Acid Cultures and Yoghurt Bacteria

Measuring the viability of probiotic microorganisms in food products using plate count methodology is a common practice due to the simplicity (ease of performance), inexpensive and routine testing characters ofthis method. In present study, the suitability of de man rogosa and sharpe agar (MRS) bile agar medium forthe selective enumeration of mixed probiotic bacteria (Lactobacillus ...

Studies of Agar-digesting Bacteria.